Measuring a Transfer Curve
To measure a point on the transfer curve of an inverter I (input A, output Z):
- Construct a “fixed drive” (with reporter)
- a constitutive promoter with output protein A
- measure reporter signal ? fA
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- Construct “fixed drive” + I (with reporter)
- measure reporter signal ? fZ
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Result: point (fA, fZ) on transfer curve of I